CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.47.10
Functional Family 3-ketoacyl-CoA thiolase, peroxisomal

Enzyme Information

2.3.1.16
Acetyl-CoA C-acyltransferase.
based on mapping to UniProt P27796
Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.
-!- The enzyme, found in both eukaryotes and in prokaryotes, is involved in degradation pathways such as fatty acid beta-oxidation. -!- The enzyme acts on 3-oxoacyl-CoAs to produce acetyl-CoA and an acyl- CoA shortened by two carbon atoms. -!- The reaction starts with the acylation of a nucleophilic cysteine at the active site by a 3-oxoacyl-CoA, with the concomitant release of acetyl-CoA. -!- In the second step the acyl group is transferred to CoA. -!- Most enzymes have a broad substrate range for the 3-oxoacyl-CoA. -!- cf. EC 2.3.1.9.

UniProtKB Entries (1)

P27796
THIK_YEAST
Saccharomyces cerevisiae S288C
3-ketoacyl-CoA thiolase, peroxisomal

PDB Structure

PDB 1AFW
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
The 1.8 A crystal structure of the dimeric peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae: implications for substrate binding and reaction mechanism.
Mathieu, M., Modis, Y., Zeelen, J.P., Engel, C.K., Abagyan, R.A., Ahlberg, A., Rasmussen, B., Lamzin, V.S., Kunau, W.H., Wierenga, R.K.
J.Mol.Biol.