Structural alignments for each cluster are annotated as follows.

• ligand-binding residues = green
• Conservation (blue) is calculated using the algorithm scorecons. The higher the degree of conservation, the darker the blue

Catalytic residues are coloured according to EC number clustering. This means that the catalytic residues of domains that have been allocated the same EC number are annotated with the same colour (red, pink, orange etc)

Cluster 1

• 2.5.1.1, 2.5.1.10 cluster.
• 2.5.1.-, 2.5.1.1, 2.5.1.29, 2.5.1.10 cluster.
• 2.5.1.29 cluster.
• 2.5.1.1, 2.5.1.10, 2.5.1.29 cluster.

2DSEC plot

• 2DSEC plot - domains from different EC clusters - catalytic residues highlighted.