CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.20 | Alpha-Beta Barrel |
|
3.20.20 | TIM Barrel |
|
3.20.20.70 | Aldolase class I |
Domain Context
CATH Clusters
| Superfamily | Aldolase class I |
| Functional Family |
Enzyme Information
| 4.1.3.44 |
tRNA 4-demethylwyosine synthase (AdoMet-dependent).
based on mapping to UniProt Q57705
N(1)-methylguanine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + L-methionine + 5'-deoxyadenosine + CO(2) + H(2)O.
-!- This enzyme, which is a member of the superfamily of S-adenosyl-L- methionine-dependent radical (radical AdoMet) enzymes, binds two [4Fe-4S] clusters. -!- Carbons C(2) and C(3) from pyruvate are incorporated into 4-demethylwyosine. -!- The enzyme is found in eukaryotes where it is part of the pathway for wybutosine synthesis, and in archaea, where it is involved in the biosynthesis of archaeal wye bases, such as wyosine, isowyosine, and methylwyosine.
|
UniProtKB Entries (1)
| Q57705 |
TYW1_METJA
Methanocaldococcus jannaschii DSM 2661
S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
|
PDB Structure
| PDB | 6DJT |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Biochemical and Structural Characterization of a Schiff Base in the Radical-Mediated Biosynthesis of 4-Demethylwyosine by TYW1.
J. Am. Chem. Soc.
|
