CATH Classification

Domain Context

CATH Clusters

Superfamily Ubiquitin Conjugating Enzyme
Functional Family Ubiquitin-conjugating enzyme E2 N

Enzyme Information

2.3.2.23
E2 ubiquitin-conjugating enzyme.
based on mapping to UniProt P61088
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine.
-!- The E2 ubiquitin-conjugating enzyme acquires the activated ubquitin from the E1 ubiquitin-activating enzyme (EC 6.2.1.45) and binds it via a transthioesterification reaction to itself. -!- In the human enzyme the catalytic center is located at Cys-87 where ubiquitin is bound via its C-terminal glycine in a thioester linkage. -!- Formerly EC 6.3.2.19.

UniProtKB Entries (2)

P61088
UBE2N_HUMAN
Homo sapiens
Ubiquitin-conjugating enzyme E2 N
Q6IWL4
TRAF6_DANRE
Danio rerio
TNF receptor-associated factor 6

PDB Structure

PDB 5VO0
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
The activity of TRAF RING homo- and heterodimers is regulated by zinc finger 1.
Middleton, A.J., Budhidarmo, R., Das, A., Zhu, J., Foglizzo, M., Mace, P.D., Day, C.L.
Nat Commun