CATH Classification

Domain Context

CATH Clusters

Superfamily Neutral Protease Domain 2
Functional Family Thermolysin

Enzyme Information

3.4.24.27
Thermolysin.
based on mapping to UniProt P00800
Preferential cleavage: Xaa-|-Leu > Xaa-|-Phe.
-!- A thermostable extracellular metalloendopeptidase containing four calcium ions. -!- Enzymes that may be species variants of thermolysin are reported from Micrococcus caseolyticus and Aspergillus oryzae. -!- Closely related but distinct enzymes are aeromonolysin, pseudolysin, bacillolysin, aureolysin and mycolysin. -!- Belongs to peptidase family M4. -!- Formerly EC 3.4.24.4.

UniProtKB Entries (1)

P00800
THER_BACTH
Bacillus thermoproteolyticus
Thermolysin

PDB Structure

PDB 5K7T
External Links
Method ELECTRON CRYSTALLOGRAPHY
Organism
Primary Citation
Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.
de la Cruz, M.J., Hattne, J., Shi, D., Seidler, P., Rodriguez, J., Reyes, F.E., Sawaya, M.R., Cascio, D., Weiss, S.C., Kim, S.K., Hinck, C.S., Hinck, A.P., Calero, G., Eisenberg, D., Gonen, T.
Nat. Methods