CATH Classification

Domain Context

CATH Clusters

Superfamily 3.90.940.10
Functional Family

Enzyme Information

2.7.7.6
DNA-directed RNA polymerase.
based on mapping to UniProt Q8RQE7
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
-!- Catalyzes DNA-template-directed extension of the 3'-end of an RNA strand by one nucleotide at a time. -!- Can initiate a chain de novo. -!- In eukaryotes three forms of the enzyme have been distinguished on the basis of sensitivity of alpha-amanitin, and the type of RNA synthesized. -!- See also EC 2.7.7.19 and EC 2.7.7.48.

UniProtKB Entries (2)

Q9Z9H6
RPOA_THETH
Thermus thermophilus
DNA-directed RNA polymerase subunit alpha
Q8RQE9
RPOB_THET8
Thermus thermophilus HB8
DNA-directed RNA polymerase subunit beta

PDB Structure

PDB 4Q5S
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural basis of transcription initiation by bacterial RNA polymerase holoenzyme.
Basu, R.S., Warner, B.A., Molodtsov, V., Pupov, D., Esyunina, D., Fernandez-Tornero, C., Kulbachinskiy, A., Murakami, K.S.
J.Biol.Chem.