CATH Classification

Domain Context

CATH Clusters

Superfamily Ubiquitin Conjugating Enzyme
Functional Family Ubiquitin-conjugating enzyme E2 L3

Enzyme Information

2.3.2.23
E2 ubiquitin-conjugating enzyme.
based on mapping to UniProt P68036
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine.
-!- The E2 ubiquitin-conjugating enzyme acquires the activated ubquitin from the E1 ubiquitin-activating enzyme (EC 6.2.1.45) and binds it via a transthioesterification reaction to itself. -!- In the human enzyme the catalytic center is located at Cys-87 where ubiquitin is bound via its C-terminal glycine in a thioester linkage. -!- Formerly EC 6.3.2.19.

UniProtKB Entries (3)

P68036
UB2L3_HUMAN
Homo sapiens
Ubiquitin-conjugating enzyme E2 L3
Q3YTH2
OSPG_SHISS
Shigella sonnei Ss046
Protein kinase OspG
P0CG63
UBI4P_YEAST
Saccharomyces cerevisiae S288C
Polyubiquitin

PDB Structure

PDB 4Q5H
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural Basis for the Inhibition of Host Protein Ubiquitination by Shigella Effector Kinase OspG.
Grishin, A.M., Condos, T.E., Barber, K.R., Campbell-Valois, F.X., Parsot, C., Shaw, G.S., Cygler, M.
Structure