CATH Domain 4gtaA00
|3.30.1360||Gyrase A; domain 2|
|Functional Family||Flavin-dependent thymidylate synthase|
Thymidylate synthase (FAD).
based on mapping to UniProt Q9WYT0
5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP(+).
-!- Contains FAD. -!- All thymidylate synthases catalyze a reductive methylation involving the transfer of the methylene group of 5,10-methylenetetrahydrofolate to the C5-position of dUMP and a two electron reduction of the methylene group to a methyl group. -!- Unlike the classical thymidylate synthase, ThyA (EC 184.108.40.206), which uses folate as both a 1-carbon donor and a source of reducing equivalents, this enzyme uses a flavin coenzyme as a source of reducing equivalents, which are derived from NADPH.
UniProtKB Entries (1)
Thermotoga maritima MSB8
Flavin-dependent thymidylate synthase
Folate binding site of flavin-dependent thymidylate synthase.