CATH Classification

Domain Context

CATH Clusters

Superfamily Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A
Functional Family

Enzyme Information

2.4.1.266
Glucosyl-3-phosphoglycerate synthase.
based on mapping to UniProt P9WMW9
NDP-glucose + 3-phospho-D-glycerate = NDP + 2-O-(alpha-D-glucopyranosyl)- 3-phospho-D-glycerate.
-!- The enzyme is involved in biosynthesis of 2-O-(alpha-D- glucopyranosyl)-D-glycerate via the two-step pathway in which glucosyl-3-phosphoglycerate synthase catalyzes the conversion of GDP- glucose and 3-phospho-D-glycerate into 2-O-(alpha-D-glucopyranosyl)- 3-phospho-D-glycerate, which is then converted to 2-O-(alpha-D- glucopyranosyl)-D-glycerate by EC 3.1.3.85. -!- The activity is dependent on divalent cations (Mn(2+), Co(2+), or Mg(2+)). -!- The enzyme from Persephonella marina shows moderate flexibility on the sugar donor concerning the nucleotide moiety (UDP-glucose, ADP- glucose, GDP-glucose) but is strictly specific for glucose. -!- The enzyme is also strictly specific for 3-phospho-D-glycerate as acceptor. -!- The enzyme from Methanococcoides burtonii is strictly specific for GDP-glucose and 3-phospho-D-glycerate. -!- This enzyme catalyzes the first glucosylation step in methylglucose lipopolysaccharide biosynthesis in mycobacteria.

UniProtKB Entries (1)

P9WMW9
GPGS_MYCTU
Mycobacterium tuberculosis H37Rv
Glucosyl-3-phosphoglycerate synthase

PDB Structure

PDB 4DE7
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Mechanistic insights into the retaining glucosyl-3-phosphoglycerate synthase from mycobacteria.
Urresti, S., Albesa-Jove, D., Schaeffer, F., Pham, H.T., Kaur, D., Gest, P., van der Woerd, M.J., Carreras-Gonzalez, A., Lopez-Fernandez, S., Alzari, P.M., Brennan, P.J., Jackson, M., Guerin, M.E.
J.Biol.Chem.