CATH Classification

Domain Context

CATH Clusters

Superfamily Peptidase C12, ubiquitin carboxyl-terminal hydrolase
Functional Family Ubiquitin carboxyl-terminal hydrolase

Enzyme Information

3.4.19.12
Ubiquitinyl hydrolase 1.
based on mapping to UniProt P09936
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
-!- Links to polypeptides smaller than 60 residues are hydrolyzed more readily than those to larger polypeptides. -!- Isoforms exist with quantitatively different specificities among the best known being UCH-L1 and UCH-L3, major proteins of the brain of mammals. -!- Inhibited by ubiquitin aldehyde (in which Gly76 is replaced by aminoacetaldehyde). -!- Belongs to peptidase family C12.

UniProtKB Entries (1)

P09936
UCHL1_HUMAN
Homo sapiens
Ubiquitin carboxyl-terminal hydrolase isozyme L1

PDB Structure

PDB 3KVF
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Ubiquitin vinyl methyl ester binding orients the misaligned active site of the ubiquitin hydrolase UCHL1 into productive conformation.
Boudreaux, D.A., Maiti, T.K., Davies, C.W., Das, C.
Proc.Natl.Acad.Sci.USA