CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
1 | Mainly Alpha |
|
1.10 | Orthogonal Bundle |
|
1.10.630 | Cytochrome p450 |
|
1.10.630.10 | Cytochrome P450 |
Domain Context
CATH Clusters
| Superfamily | Cytochrome P450 |
| Functional Family | Steroid C26-monooxygenase |
Enzyme Information
| 1.14.15.29 |
Cholest-4-en-3-one 26-monooxygenase ((25S)-3-oxocholest-4-en-26-oate forming).
based on mapping to UniProt P9WPP1
Cholest-4-en-3-one + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H(+) + 3 O(2) = (25S)-3-oxocholest-4-en-26-oate + 6 oxidized ferredoxin [iron- sulfur] cluster + 4 H(2)O.
-!- A cytochrome P450 (heme-thiolate) protein found in several bacterial pathogens. -!- The enzyme is involved in degradation of the host's cholesterol. -!- It catalyzes the hydroxylation of the C-26 carbon, followed by oxidation of the alcohol to the carboxylic acid via the aldehyde intermediate, initiating the degradation of the alkyl side-chain of cholesterol. -!- The products are exclusively in the (25S) conformation. -!- The enzyme also accepts cholesterol as a substrate. -!- Cf. EC 1.14.15.28. -!- Formerly EC 1.14.13.141.
|
UniProtKB Entries (1)
| P9WPP1 |
CP125_MYCTU
Mycobacterium tuberculosis H37Rv
Steroid C26-monooxygenase
|
PDB Structure
| PDB | 3IW0 |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
The Structure of Mycobacterium tuberculosis CYP125: molecular basis for cholesterol binding in a P450 needed for host infection.
J.Biol.Chem.
|
