CATH Classification

Domain Context

CATH Clusters

Superfamily Hotdog Thioesterase
Functional Family

Enzyme Information

4.2.1.59
3-hydroxyacyl-[acyl-carrier-protein] dehydratase.
based on mapping to UniProt A1VXZ7
A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl- carrier protein] + H(2)O.
-!- This enzyme is responsible for the dehydration step of the dissociated (type II) fatty-acid biosynthesis system that occurs in plants and bacteria. -!- The enzyme uses fatty acyl thioesters of ACP in vivo. -!- Different forms of the enzyme may have preferences for substrates with different chain length. -!- For example, the activity of FabZ, the ubiquitous enzyme in bacteria, decreases with increasing chain length. -!- Gram-negative bacteria that produce unsaturated fatty acids, such as Escherichia coli, have another form (FabA) that prefers intermediate chain length, and also catalyzes EC 5.3.3.14. -!- Despite the differences both forms can catalyze all steps leading to the synthesis of palmitate (C16:0). -!- FabZ, but not FabA, can also accept unsaturated substrates. -!- Formerly EC 4.2.1.58, EC 4.2.1.60 and EC 4.2.1.61.

UniProtKB Entries (1)

A1VXZ7
FABZ_CAMJJ
Campylobacter jejuni subsp. jejuni 81-176
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ

PDB Structure

PDB 3D6X
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Campylobacter jejuni fatty acid synthase II: structural and functional analysis of beta-hydroxyacyl-ACP dehydratase (FabZ).
Kirkpatrick, A.S., Yokoyama, T., Choi, K.J., Yeo, H.J.
Biochem.Biophys.Res.Commun.