CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.20 | Alpha-Beta Barrel |
|
3.20.20 | TIM Barrel |
|
3.20.20.100 | NADP-dependent oxidoreductase domain |
Domain Context
CATH Clusters
| Superfamily | NADP-dependent oxidoreductase domain |
| Functional Family | Aldo-keto reductase family 1 member C3 |
Enzyme Information
| 1.3.1.3 |
Delta(4)-3-oxosteroid 5-beta-reductase.
based on mapping to UniProt P51857
(1) 5-beta-cholestan-3-one + NADP(+) = cholest-4-en-3-one + NADPH. (2) 17,21-dihydroxy-5-beta-pregnane-3,11,20-trione + NADP(+) = cortisone + NADPH.
-!- The enzyme from human efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites; it can also act on aldosterone, corticosterone and cortisol, but to a lesser extent. -!- The bile acid intermediates 7-alpha,12-alpha-dihydroxy-4-cholesten-3- one and 7-alpha-hydroxy-4-cholesten-3-one can also act as substrates.
|
UniProtKB Entries (1)
| P51857 |
AK1D1_HUMAN
Homo sapiens
3-oxo-5-beta-steroid 4-dehydrogenase
|
PDB Structure
| PDB | 3CAV |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
The crystal structure of human Delta4-3-ketosteroid 5beta-reductase defines the functional role of the residues of the catalytic tetrad in the steroid double bond reduction mechanism.
Biochemistry
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