CATH Classification

Domain Context

CATH Clusters

Superfamily Alpha-ketoglutarate-dependent dioxygenase AlkB-like
Functional Family Alpha-ketoglutarate-dependent dioxygenase AlkB

Enzyme Information

1.14.11.33
DNA oxidative demethylase.
based on mapping to UniProt P05050
DNA-base-CH(3) + 2-oxoglutarate + O(2) = DNA-base + formaldehyde + succinate + CO(2).
-!- Activity is slightly stimulated by ascorbate. -!- Catalyzes oxidative demethylation of the DNA base lesions N(1)- methyladenine, N(3)-methylcytosine, N(1)-methylguanine, and N(3)- methylthymine. -!- It works better on single-stranded DNA (ssDNA) and is capable of repairing damaged bases in RNA.

UniProtKB Entries (1)

P05050
ALKB_ECOLI
Escherichia coli K-12
Alpha-ketoglutarate-dependent dioxygenase AlkB

PDB Structure

PDB 3BKZ
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Crystal structures of DNA/RNA repair enzymes AlkB and ABH2 bound to dsDNA
Yang, C.-G., Yi, C., Duguid, E.M., Sullivan, C.T., Jian, X., Rice, P.A., He, C.
Nature