CATH Classification

Domain Context

CATH Clusters

Superfamily Peptidase C65 Otubain, subdomain 1
Functional Family Ubiquitin thioesterase OTUB1

Enzyme Information

3.4.19.12
Ubiquitinyl hydrolase 1.
based on mapping to UniProt Q96FW1
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
-!- Links to polypeptides smaller than 60 residues are hydrolyzed more readily than those to larger polypeptides. -!- Isoforms exist with quantitatively different specificities among the best known being UCH-L1 and UCH-L3, major proteins of the brain of mammals. -!- Inhibited by ubiquitin aldehyde (in which Gly76 is replaced by aminoacetaldehyde). -!- Belongs to peptidase family C12.

UniProtKB Entries (1)

Q96FW1
OTUB1_HUMAN
Homo sapiens
Ubiquitin thioesterase OTUB1

PDB Structure

PDB 2ZFY
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structural basis and specificity of human otubain 1-mediated deubiquitination.
Edelmann, M.J., Iphofer, A., Akutsu, M., Altun, M., di Gleria, K., Kramer, H.B., Fiebiger, E., Dhe-Paganon, S., Kessler, B.M.
Biochem.J.