CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.50.1400
Functional Family Sirohydrochlorin cobaltochelatase

Enzyme Information

4.99.1.3
Sirohydrochlorin cobaltochelatase.
based on mapping to UniProt O29537
Cobalt-sirohydrochlorin + 2 H(+) = sirohydrochlorin + Co(2+).
-!- This enzyme is a type II chelatase, being either a monomer (CbiX) or a homodimer (CibK) and being ATP-independent. -!- CbiK from Salmonella enterica uses precorrin-2 as the substrate to yield cobalt-precorrin-2. -!- The enzyme contains two histidines at the active site that are thought to be involved in the deprotonation of the tetrapyrrole substrate as well as in metal binding. -!- CbiX from Bacillus megaterium inserts cobalt at the level of sirohydrochlorin (factor-II) rather than precorrin-2.

UniProtKB Entries (1)

O29537
CBIX_ARCFU
Archaeoglobus fulgidus DSM 4304
Sirohydrochlorin cobaltochelatase

PDB Structure

PDB 2XWQ
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Evolution in a Family of Chelatases Facilitated by the Introduction of Active Site Asymmetry and Protein Oligomerization.
Romao, C.V., Ladakis, D., Lobo, S.A., Carrondo, M.A., Brindley, A.A., Deery, E., Matias, P.M., Pickersgill, R.W., Saraiva, L.M., Warren, M.J.
Proc.Natl.Acad.Sci.USA