CATH Domain 2lajA01

CATH Classification

Level	CATH Code	Description
	2	Mainly Beta
	2.20	Single Sheet
	2.20.70	Ubiquitin Ligase Nedd4; Chain: W;
	2.20.70.10

Domain Context

CATH Clusters

Superfamily	2.20.70.10
Functional Family	E3 ubiquitin-protein ligase NEDD4-like protein

Enzyme Information

2.3.2.26

HECT-type E3 ubiquitin transferase.

based on mapping to UniProt Q96PU5

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine.

-!- In the first step the enzyme transfers ubiquitin from the E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) to a cysteine residue in its HECT domain (which is located in the C-terminal region), forming a thioester bond. -!- In a subsequent step the enzyme transfers the ubiquitin to an acceptor protein, resulting in the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon- amino group of an L-lysine residue of the acceptor protein. -!- Cf. EC 2.3.2.27 and EC 2.3.2.31.

UniProtKB Entries (1)

P84022

SMAD3_HUMAN

Homo sapiens

Mothers against decapentaplegic homolog 3

PDB Structure

PDB	2LAJ
External Links	PDBSum Proteopedia
Method	SOLUTION NMR
Organism
Primary Citation	A Smad action turnover switch operated by WW domain readers of a phosphoserine code. Aragon, E., Goerner, N., Zaromytidou, A.I., Xi, Q., Escobedo, A., Massague, J., Macias, M.J. Genes Dev.