CATH Classification

Domain Context

CATH Clusters

Superfamily tRNA wybutosine-synthesizing-like
Functional Family tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase 1

Enzyme Information

2.1.1.282
tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))- methyltransferase.
based on mapping to UniProt O58796
S-adenosyl-L-methionine + 7-((3S)-(3-amino-3-carboxypropyl))-4- demethylwyosine(37) in tRNA(Phe) = S-adenosyl-L-homocysteine + 7-((3S)- (3-amino-3-carboxypropyl))wyosine(37) in tRNA(Phe).
-!- The enzyme is involved in the biosynthesis of hypermodified tricyclic bases found at position 37 of certain tRNAs. -!- These modifications are important for translational reading-frame maintenance. -!- The enzyme is found in all eukaryotes and in some archaea, but not in bacteria. -!- The eukaryotic enzyme is involved in the biosynthesis of wybutosine.

UniProtKB Entries (1)

O58796
TYW31_PYRHO
Pyrococcus horikoshii OT3
TRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase 1

PDB Structure

PDB 2IT2
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structure of PH1069 protein from Pyrococcus horikoshii
Lokanath, N.K., Kunishima, N.
To be Published