CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.10 | Roll |
|
3.10.110 | Ubiquitin Conjugating Enzyme |
|
3.10.110.10 | Ubiquitin Conjugating Enzyme |
Domain Context
CATH Clusters
| Superfamily | Ubiquitin Conjugating Enzyme |
| Functional Family | NEDD8-conjugating enzyme UBE2F |
Enzyme Information
| 2.3.2.32 |
Cullin-RING-type E3 NEDD8 transferase.
based on mapping to UniProt Q969M7
S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]- L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N(6)-[NEDD8- protein]-yl-[cullin]-L-lysine.
-!- Some RING-type E3 ubiquitin transferase (EC 2.3.2.27) are not able to bind a substrate protein directly. -!- Instead, they form a complex with a cullin scaffold protein and a substrate recognition module, which is named CRL for Cullin-RING- Ligase. -!- The cullin protein needs to be activated by the ubiquitin-like protein NEDD8 in a process known as neddylation. -!- The transfer of NEDD8 from a NEDD8-specific E2 enzyme onto the cullin protein is a secondary function of the RING-type E3 ubiquitin transferase in the CRL complex. -!- The process requires auxiliary factors that belong to the DCN1 (defective in cullin neddylation 1) family.
|
UniProtKB Entries (1)
| Q969M7 |
UBE2F_HUMAN
Homo sapiens
NEDD8-conjugating enzyme UBE2F
|
PDB Structure
| PDB | 2EDI |
| External Links | |
| Method | SOLUTION NMR |
| Organism | |
| Primary Citation |
Solution structure of the UQ_con domain from human NEDD8-conjugating enzyme NCE2
To be Published
|
