CATH Classification

Domain Context

CATH Clusters

Superfamily 3.30.70.3180
Functional Family

Enzyme Information

2.1.1.148
Thymidylate synthase (FAD).
based on mapping to UniProt P9WG57
5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP(+).
-!- Contains FAD. -!- All thymidylate synthases catalyze a reductive methylation involving the transfer of the methylene group of 5,10-methylenetetrahydrofolate to the C5-position of dUMP and a two electron reduction of the methylene group to a methyl group. -!- Unlike the classical thymidylate synthase, ThyA (EC 2.1.1.45), which uses folate as both a 1-carbon donor and a source of reducing equivalents, this enzyme uses a flavin coenzyme as a source of reducing equivalents, which are derived from NADPH.

UniProtKB Entries (1)

P9WG57
THYX_MYCTU
Mycobacterium tuberculosis H37Rv
Flavin-dependent thymidylate synthase

PDB Structure

PDB 2AF6
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structure of the Mycobacterium tuberculosis Flavin Dependent Thymidylate Synthase (MtbThyX) at 2.0A Resolution.
Sampathkumar, P., Turley, S., Ulmer, J.E., Rhie, H.G., Sibley, C.H., Hol, W.G.
J.Mol.Biol.