CATH Classification

Domain Context

CATH Clusters

Superfamily NAD(P)-binding Rossmann-like Domain
Functional Family peroxisomal multifunctional enzyme type 2

Enzyme Information
3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase.
based on mapping to UniProt P51659
(24R,25R)-3-alpha,7-alpha,12-alpha,24-tetrahydroxy-5-beta-cholestanoyl- CoA = (24E)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl- CoA + H(2)O.
-!- Forms part of the rat peroxisomal multifunctional enzyme perMFE-2, which also exhibits a dehydrogenase activity. -!- Involved in the beta-oxidation of the cholesterol side chain in the cholic-acid biosynthesis pathway.
Enoyl-CoA hydratase 2.
based on mapping to UniProt P51659
(3R)-3-hydroxyacyl-CoA = (2E)-2-enoyl-CoA + H(2)O.
-!- This enzyme catalyzes a hydration step in peroxisomal beta-oxidation. -!- The human multifunctional enzyme type 2 (MFE-2) is a 79000 Da enzyme composed of three functional units: (3R)-hydroxyacyl-CoA dehydrogenase, 2-enoyl-CoA hydratase 2 and sterol carrier protein 2-like units. -!- The enzymes from Aeromonas caviae and Arabidopsis thaliana are monofunctional enzymes. -!- 2-enoyl-CoA hydratase 3 from Candida tropicalis is a part from multifunctional enzyme type 2.
(3R)-hydroxyacyl-CoA dehydrogenase.
based on mapping to UniProt P51659
(R)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH.

UniProtKB Entries (1)

Homo sapiens
Peroxisomal multifunctional enzyme type 2

PDB Structure

External Links
Organism Escherichia
Primary Citation
Crystal Structure Of Human 17-Beta-Hydroxysteroid Dehydrogenase Type 4 In Complex With NAD
Lukacik, P., Shafqat, N., Kavanagh, K., Bray, J., von Delft, F., Edwards, A., Arrowsmith, C., Sundstrom, M., Oppermann, U.
To be Published