CATH Classification

Domain Context

CATH Clusters

Superfamily Ubiquitin-activating enzyme E1, SCCH domain
Functional Family Ubiquitin-activating enzyme E1 1

Enzyme Information
E1 ubiquitin-activating enzyme.
based on mapping to UniProt Q02053
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine.
-!- Catalyzes the ATP-dependent activation of ubiquitin through the formation of a thioester bond between the C-terminal glycine of ubiquitin and the sulfhydryl side group of a cysteine residue in the E1 protein. -!- The two-step reaction consists of the ATP-dependent formation of an E1-ubiquitin adenylate intermediate in which the C-terminal glycine of ubiquitin is bound to AMP via an acyl-phosphate linkage, then followed by the conversion to an E1-ubiquitin thioester bond via the cysteine residue on E1 in the second step. -!- Formerly EC and EC

UniProtKB Entries (1)

Mus musculus
Ubiquitin-like modifier-activating enzyme 1

PDB Structure

External Links
Organism Escherichia
Primary Citation
Crystal structure of a fragment of mouse ubiquitin-activating enzyme.
Szczepanowski, R.H., Filipek, R., Bochtler, M.