CATH Classification

Domain Context

CATH Clusters

Superfamily Golgi alpha-mannosidase II
Functional Family Sucrose isomerase

Enzyme Information

3.2.1.10
Oligo-1,6-glucosidase.
based on mapping to UniProt P21332
Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.
-!- This enzyme, like EC 3.2.1.33, can release an alpha-1->6-linked glucose, whereas the shortest chain that can be released by EC 3.2.1.41, EC 3.2.1.142 and EC 3.2.1.68 is maltose. -!- It also hydrolyzes isomaltulose (palatinose), isomaltotriose and panose, but has no action on glycogen or phosphorylase limit dextrin. -!- The enzyme from intestinal mucosa is a single polypeptide chain that also catalyzes the reaction of EC 3.2.1.48. -!- Differs from EC 3.2.1.33 in its preference for short-chain substrates and in its not requiring the 6-glucosylated residue to be at a branch point, i.e. linked at both C-1 and C-4.

UniProtKB Entries (1)

P21332
O16G_BACCE
Bacillus cereus
Oligo-1,6-glucosidase

PDB Structure

PDB 1UOK
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
The refined crystal structure of Bacillus cereus oligo-1,6-glucosidase at 2.0 A resolution: structural characterization of proline-substitution sites for protein thermostabilization.
Watanabe, K., Hata, Y., Kizaki, H., Katsube, Y., Suzuki, Y.
J.Mol.Biol.