CATH Classification

Domain Context

CATH Clusters

Superfamily 2-methylcitrate dehydratase PrpD
Functional Family 2-methylcitrate dehydratase

Enzyme Information
Aconitate hydratase.
based on mapping to UniProt P77243
Citrate = isocitrate.
-!- Besides interconverting citrate and cis-aconitate, it also interconverts cis-aconitate with isocitrate and, hence, interconverts citrate and isocitrate. -!- The equilibrium mixture is 91% citrate, 6% isocitrate and 3% aconitate. -!- Cis-aconitate is used to designate the isomer (Z)-prop-1-ene-1,2,3- tricarboxylate. -!- Formerly EC
2-methylcitrate dehydratase.
based on mapping to UniProt P77243
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3- tricarboxylate + H(2)O.
-!- Specific for (2S,3S)-methylcitrate, showing no activity with (2R,3S)- methylcitrate. -!- Can also use cis-aconitate as a substrate but more slowly. -!- Both this enzyme and EC are required to complete the isomerization of (2S,3S)-methylcitrate to (2R,3S)-2-methylisocitrate.

UniProtKB Entries (1)

Escherichia coli K-12
2-methylcitrate dehydratase

PDB Structure

External Links
Organism Escherichia
Primary Citation
Crystal structure of 2-methylcitrate dehydratase
Rajashankar, K.R., Kniewel, R., Solorzano, V., Lima, C.D.
To be Published