CATH Classification

Domain Context

CATH Clusters

Superfamily 3.30.70.270
Functional Family Genome polyprotein

Enzyme Information

2.7.7.48
RNA-directed RNA polymerase.
based on mapping to UniProt P19711
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
-!- Catalyzes RNA-template-directed extension of the 3'-end of an RNA strand by one nucleotide at a time. -!- Can initiate a chain de novo. -!- See also EC 2.7.7.6.
3.4.21.113
Pestivirus NS3 polyprotein peptidase.
based on mapping to UniProt P19711
Leu is conserved at position P1 for all four cleavage sites. Alanine is found at position P1' of the NS4A-NS4B cleavage site, whereas serine is found at position P1' of the NS3-NS4A, NS4B-NS5A and NS5A-NS5B cleavage sites.
-!- The polyprotein of noncytopathogenic pestiviruses is cleaved co- and post-translationally into at least 11 proteins (N(pro), C, E(rns), E1, E2, p7, NS2-3, NS4A, NS4B, NS5A, and NS5B). -!- The genomes of cytopathogenic pestivirus strains express at least one additional protein, called NS3 (p80). -!- This enzyme, which resides in the N-terminal region of NS3 (nonstructural protein 3), is essential for generation of its own C-terminus and for processing of the downstream cleavage sites, leading to the release of the pestivirus nonstructural proteins NS4A, NS4B, NS5A and NS5B. -!- Belongs to peptidase family S31.
3.6.4.13
RNA helicase.
based on mapping to UniProt P19711
ATP + H(2)O = ADP + phosphate.
-!- RNA helicases utilize the energy from ATP hydrolysis to unwind RNA. -!- Some of them unwind RNA with a 3' to 5' polarity, other show 5' to 3' polarity. -!- Some helicases unwind DNA as well as RNA. -!- May be identical with EC 3.6.4.12 (DNA helicase).
3.6.1.15
Nucleoside-triphosphate phosphatase.
based on mapping to UniProt P19711
NTP + H(2)O = NDP + phosphate.
-!- The enzyme is found in eukaryotes and thermophilic bacteria, but appears to be absent from mesophilic bacteria. -!- Also hydrolyzes nucleoside diphosphates, thiamine diphosphate and FAD. -!- The enzyme from the plant Pisum sativum (garden pea) is regulated by calmodulin.
3.4.22.-
Cysteine endopeptidases.
based on mapping to UniProt P19711

UniProtKB Entries (1)

P19711
POLG_BVDVN
Bovine viral diarrhea virus 1-NADL
Genome polyprotein

PDB Structure

PDB 1S4F
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
The structure of the RNA-dependent RNA polymerase from bovine viral diarrhea virus establishes the role of GTP in de novo initiation.
Choi, K.H., Groarke, J.M., Young, D.C., Kuhn, R.J., Smith, J.L., Pevear, D.C., Rossmann, M.G.
Proc.Natl.Acad.Sci.Usa