CATH Classification

Domain Context

CATH Clusters

Superfamily 3.30.1360.170
Functional Family Flavin-dependent thymidylate synthase

Enzyme Information
Thymidylate synthase (FAD).
based on mapping to UniProt Q9WYT0
5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP(+).
-!- Contains FAD. -!- All thymidylate synthases catalyze a reductive methylation involving the transfer of the methylene group of 5,10-methylenetetrahydrofolate to the C5-position of dUMP and a two electron reduction of the methylene group to a methyl group. -!- Unlike the classical thymidylate synthase, ThyA (EC, which uses folate as both a 1-carbon donor and a source of reducing equivalents, this enzyme uses a flavin coenzyme as a source of reducing equivalents, which are derived from NADPH.

UniProtKB Entries (1)

Thermotoga maritima MSB8
Flavin-dependent thymidylate synthase

PDB Structure

PDB 1O25
External Links
Organism Escherichia
Primary Citation
Functional Analysis of Substrate and Cofactor Complex Structures of a Thymidylate Synthase-Complementing Protein
Mathews, I.I., Deacon, A.M., Canaves, J.M., McMullan, D., Lesley, S.A., Agarwalla, S., Kuhn, P.