CATH Classification

Domain Context

CATH Clusters

Superfamily Nitric Oxide Synthase;Heme Domain;Chain A domain 2
Functional Family Endothelial nitric oxide synthase

Enzyme Information
Nitric-oxide synthase (NADPH).
based on mapping to UniProt P29474
2 L-arginine + 3 NADPH + 4 O(2) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O.
-!- The enzyme consists of linked oxygenase and reductase domains. -!- The eukaryotic enzyme binds FAD, FMN, heme (iron protoporphyrin IX) and tetrahydrobiopterin, and its two domains are linked via a regulatory calmodulin-binding domain. -!- Upon calcium-induced calmodulin binding, the reductase and oxygenase domains form a complex, allowing electrons to flow from NADPH via FAD and FMN to the active center. -!- The reductase domain of the enzyme from the bacterium Sorangium cellulosum utilizes a [2Fe-2S] cluster to transfer the electrons from NADPH to the active center. -!- Cf. EC

UniProtKB Entries (1)

Homo sapiens
Nitric oxide synthase, endothelial

PDB Structure

External Links
Organism Escherichia
Primary Citation
Conformational Changes in Nitric Oxide Synthases Induced by Chlorzoxazone and Nitroindazoles: Crystallographic and Computational Analyses of Inhibitor Potency
Rosenfeld, R.J., Garcin, E.D., Panda, K., Andersson, G., Aberg, A., Wallace, A.V., Morris, G.M., Olson, A.J., Stuehr, D.J., Tainer, J.A., Getzoff, E.D.