CATH Domain 1jwjB02
|3.90.440||Nitric Oxide Synthase;Heme Domain; Chain A, domain 2|
|3.90.440.10||Nitric Oxide Synthase;Heme Domain;Chain A domain 2|
|Superfamily||Nitric Oxide Synthase;Heme Domain;Chain A domain 2|
|Functional Family||Nitric oxide synthase, inducible|
Nitric-oxide synthase (NADPH).
based on mapping to UniProt P29477
2 L-arginine + 3 NADPH + 4 O(2) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O.
-!- The enzyme consists of linked oxygenase and reductase domains. -!- The eukaryotic enzyme binds FAD, FMN, heme (iron protoporphyrin IX) and tetrahydrobiopterin, and its two domains are linked via a regulatory calmodulin-binding domain. -!- Upon calcium-induced calmodulin binding, the reductase and oxygenase domains form a complex, allowing electrons to flow from NADPH via FAD and FMN to the active center. -!- The reductase domain of the enzyme from the bacterium Sorangium cellulosum utilizes a [2Fe-2S] cluster to transfer the electrons from NADPH to the active center. -!- Cf. EC 18.104.22.168.
UniProtKB Entries (1)
Nitric oxide synthase, inducible
Structures of tetrahydrobiopterin binding-site mutants of inducible nitric oxide synthase oxygenase dimer and implicated roles of Trp457.