CATH Classification

Domain Context

CATH Clusters

Superfamily Glycosidases
Functional Family Beta-glucosidase 7

Enzyme Information

3.2.1.182
4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase.
based on mapping to UniProt P49235
(1) (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H(2)O = 2,4-dihydroxy-7-methoxy-2H-1,4- benzoxazin-3(4H)-one + D-glucose. (2) (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D- glucopyranoside + H(2)O = 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + D-glucose.
-!- The enzyme from Triticum aestivum (wheat) has a higher affinity for DIMBOA glucoside than DIBOA glucoside. -!- With Secale cereale (rye) the preference is reversed.
3.2.1.21
Beta-glucosidase.
based on mapping to UniProt P49235
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.
-!- Wide specificity for beta-D-glucosides. -!- Some examples also hydrolyze one or more of the following: beta-D- galactosides, alpha-L-arabinosides, beta-D-xylosides and beta-D- fucosides.

UniProtKB Entries (1)

P49235
HGGL1_MAIZE
Zea mays
4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1, chloroplastic

PDB Structure

PDB 1H49
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Mutational and Structural Analysis of Aglycone Specificity in Maize and Sorghum Beta-Glucosidases
Verdoucq, L., Czjzek, M., Moriniere, J., Bevan, D.R., Esen, A.
J.Biol.Chem.