CATH Classification

Domain Context

CATH Clusters

Superfamily alpha/beta hydrolase
Functional Family (S)-hydroxynitrile lyase

Enzyme Information
(S)-hydroxynitrile lyase.
based on mapping to UniProt P52705
(1) An aliphatic (S)-hydroxynitrile = cyanide + an aliphatic aldehyde or ketone. (2) An aromatic (S)-hydroxynitrile = cyanide + an aromatic aldehyde.
-!- Hydroxynitrile lyases catalyzes the the cleavage of hydroxynitriles into cyanide and the corresponding aldehyde or ketone. -!- In nature the liberation of cyanide serves as a defense mechanism against herbivores and microbial attack in plants. -!- In vitro the enzymes from Manihot esculenta and Hevea brasiliensis accept a broad range of aliphatic and aromatic carbonyl compounds as substrates and catalyze the formation of (S)-hydroxynitriles. -!- Formerly EC and EC

UniProtKB Entries (1)

Manihot esculenta
(S)-hydroxynitrile lyase

PDB Structure

PDB 1E89
External Links
Primary Citation
Mechanistic Aspects of Cyanogenesis from Active-Site Mutant Ser80Ala of Hydroxynitrile Lyase from Manihot Esculenta in Complex with Acetone Cyanohydrin
Lauble, H., Miehlich, B., Foerster, S., Wajant, H., Effenberger, F.
Protein Sci.