CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.40 | 3-Layer(aba) Sandwich |
|
3.40.930 | Mannitol-specific EII; Chain A |
|
3.40.930.10 | Mannitol-specific EII; Chain A |
Domain Context
CATH Clusters
| Superfamily | Mannitol-specific EII; Chain A |
| Functional Family | PTS system mannitol-specific transporter subunit IICBA |
Enzyme Information
| 2.7.1.197 |
Protein-N(pi)-phosphohistidine--D-mannitol phosphotransferase.
based on mapping to UniProt P00550
[Protein]-N(pi)-phospho-L-histidine + D-mannitol(Side 1) = [protein]-L- histidine + D-mannitol 1-phosphate(Side 2).
-!- This enzyme is a component (known as enzyme II) of a phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTS). -!- The system, which is found only in prokaryotes, simultaneously transports its substrate from the periplasm or extracellular space into the cytoplasm and phosphorylates it. -!- The phosphate donor, which is shared among the different systems, is a phospho-carrier protein of low molecular mass that has been phosphorylated by EC 2.7.3.9 (phosphoenolpyruvate--protein phosphotransferase). -!- Enzyme II, on the other hand, is specific for a particular substrate, although in some cases alternative substrates can be transported with lower efficiency. -!- The reaction involves a successive transfer of the phosphate group to several amino acids within the enzyme before the final transfer to the substrate. -!- Formerly EC 2.7.1.69.
|
UniProtKB Entries (1)
| P00550 |
PTM3C_ECOLI
Escherichia coli K-12
PTS system mannitol-specific EIICBA component
|
PDB Structure
| PDB | 1A3A |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | Escherichia |
| Primary Citation |
The structure of the Escherichia coli phosphotransferase IIAmannitol reveals a novel fold with two conformations of the active site.
Structure
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