CATH Classification

Domain Context

CATH Clusters

Superfamily Biosynthetic peptidoglycan transglycosylase-like
Functional Family Penicillin-binding protein 1B

Enzyme Information

3.4.16.4
Serine-type D-Ala-D-Ala carboxypeptidase.
based on mapping to UniProt P02919
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.
-!- A group of bacterial enzymes, membrane-bound. -!- Inhibited by beta-lactam antibiotics, which acylate the active site serine in the enzyme. -!- Distinct from EC 3.4.17.14. -!- Belongs to peptidase families S11, S12 and S13.
2.4.1.129
Peptidoglycan glycosyltransferase.
based on mapping to UniProt P02919
(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)- diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys- D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala- gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate.
-!- The enzyme also works when the lysine residue is replaced by meso- 2,6-diaminoheptanedioate (meso-2,6-diaminopimelate, A2pm) combined with adjacent residues through its L-center, as it is in Gram- negative and some Gram-positive organisms. -!- The undecaprenol involved is ditrans,octacis-undecaprenol. -!- Involved in the synthesis of cell-wall peptidoglycan.

UniProtKB Entries (1)

P02919
PBPB_ECOLI
Escherichia coli K-12
Penicillin-binding protein 1B

PDB Structure

PDB 5HLD
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Escherichia coli Penicillin-Binding Protein 1B: Structural Insights into Inhibition.
King, D.T., Wasney, G.A., Nosella, M., Fong, A., Strynadka, N.C.
J. Biol. Chem.