CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.630.30
Functional Family

Enzyme Information

2.3.1.271
L-glutamate-5-semialdehyde N-acetyltransferase.
based on mapping to UniProt E9P8D2
Acetyl-CoA + L-glutamate-5-semialdehyde = CoA + N-acetyl-L-glutamate 5-semialdehyde.
-!- The enzyme, characterized from the yeast Saccharomyces cerevisiae Sigma-1278b, N-acetylates L-glutamate-5-semialdehyde, an L-proline biosynthesis/utilization intermediate, into N-acetyl-L-glutamate 5-semialdehyde, an intermediate of L-arginine biosynthesis, under oxidative stress conditions. -!- Its activity results in conversion of L-proline to L-arginine, and reduction in the concentration of L-glutamate 5-semialdehyde and its equilibrium partner, (S)-1-pyrroline-5-carboxylate, which has been linked to production of reactive oxygen species stress. -!- The enzyme also acts on (S)-1-acetylazetidine-2-carboxylate, a toxic L-proline analog produced by some plants, resulting in its detoxification and conferring resistance on the yeast.

UniProtKB Entries (1)

E9P8D2
MPR1_YEASX
Saccharomyces cerevisiae
N-acetyltransferase MPR1

PDB Structure

PDB 3W6X
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural and functional analysis of the yeast N-acetyltransferase Mpr1 involved in oxidative stress tolerance via proline metabolism
Nasuno, R., Hirano, Y., Itoh, T., Hakoshima, T., Hibi, T., Takagi, H.
Proc.Natl.Acad.Sci.USA