CATH Classification

Domain Context

CATH Clusters

Superfamily Vaccinia Virus protein VP39
Functional Family tRNA wybutosine-synthesizing protein 4

Enzyme Information

2.1.1.290
tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase.
based on mapping to UniProt Q08282
S-adenosyl-L-methionine + 7-((3S)-3-amino-3-carboxypropyl)wyosine(37) in tRNA(Phe) = S-adenosyl-L-homocysteine + 7-((3S)-3-amino-3- (methoxycarbonyl)propyl)wyosine(37) in tRNA(Phe).
-!- The enzyme is found only in eukaryotes, where it is involved in the biosynthesis of wybutosine, a hypermodified tricyclic base found at position 37 of certain tRNAs. -!- The modification is important for translational reading-frame maintenance. -!- In some species that produce hydroxywybutosine the enzyme uses 7-(2-hydroxy-3-amino-3-carboxypropyl)wyosine(37) in tRNA(Phe) as substrate. -!- The enzyme also has the activity of EC 2.3.1.231.
2.3.1.231
tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)- methoxycarbonyltransferase.
based on mapping to UniProt Q08282
S-adenosyl-L-methionine + 7-((3S)-3-amino-3- (methoxycarbonyl)propyl)wyosine(37) in tRNA(Phe) + CO(2) = S-adenosyl-L- homocysteine + wybutosine(37) in tRNA(Phe).
-!- The enzyme is found only in eukaryotes, where it is involved in the biosynthesis of wybutosine, a hypermodified tricyclic base found at position 37 of certain tRNAs. -!- The modification is important for translational reading-frame maintenance. -!- In some species that produce hydroxywybutosine the enzyme uses 7-(2-hydroxy-3-amino-3-(methoxycarbonyl)propyl)wyosine(37) in tRNA(Phe) as substrate. -!- The enzyme also has the activity of EC 2.1.1.290.

UniProtKB Entries (1)

Q08282
TYW4_YEAST
Saccharomyces cerevisiae S288C
TRNA wybutosine-synthesizing protein 4

PDB Structure

PDB 2ZZK
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural basis of tRNA modification with CO2 fixation and methylation by wybutosine synthesizing enzyme TYW4.
Suzuki, Y., Noma, A., Suzuki, T., Ishitani, R., Nureki, O.
Nucleic Acids Res.