CATH Classification

Domain Context

CATH Clusters

Superfamily HUPs
Functional Family tRNA-specific 2-thiouridylase MnmA

Enzyme Information

2.8.1.13
tRNA-uridine 2-sulfurtransferase.
based on mapping to UniProt P25745
A [protein]-S-sulfanyl-L-cysteine + uridine(34) in tRNA + ATP + reduced acceptor = a [protein]-L-cysteine + 2-thiouridine(34) in tRNA + AMP + diphosphate + acceptor.
-!- The enzyme, found in bacteria, catalyzes formation of the 2-thiouridine modification in the wobble position of tRNA(Gln), tRNA(Lys) and tRNA(Glu).

UniProtKB Entries (1)

P25745
MNMA_ECOLI
Escherichia coli K-12
TRNA-specific 2-thiouridylase MnmA

PDB Structure

PDB 2DEU
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Snapshots of tRNA sulphuration via an adenylated intermediate
Numata, T., Ikeuchi, Y., Fukai, S., Suzuki, T., Nureki, O.
Nature