CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.50.970
Functional Family Pyruvate dehydrogenase E1 beta subunit

Enzyme Information

1.2.4.4
3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring).
based on mapping to UniProt P09061
3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine- residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO(2).
-!- It acts not only on 3-methyl-2-oxobutanaoate, but also on 4-methyl-2- oxopentanoate and (S)-3-methyl-2-oxopentanoate, so that it acts on the 2-oxo acids that derive from the action of transaminases on valine, leucine and isoleucine. -!- It is a component of the multienzyme 3-methyl-2-oxobutanoate dehydrogenase complex in which multiple copies of it are bound to a core of molecules of EC 2.3.1.168, which also binds multiple copies of EC 1.8.1.4. -!- It does not act on free lipoamide or lipoyllysine, but only on the lipoyllysine residue in EC 2.3.1.168. -!- Formerly EC 1.2.4.3.

UniProtKB Entries (1)

P09061
ODBB_PSEPU
Pseudomonas putida
2-oxoisovalerate dehydrogenase subunit beta

PDB Structure

PDB 1QS0
External Links
Method X-RAY DIFFRACTION
Organism Pseudomonas
Primary Citation
Crystal structure of 2-oxoisovalerate and dehydrogenase and the architecture of 2-oxo acid dehydrogenase multienzyme complexes.
Aevarsson, A., Seger, K., Turley, S., Sokatch, J.R., Hol, W.G.
Nat.Struct.Biol.