CATH Classification

Domain Context

CATH Clusters

Superfamily Collagenase (Catalytic Domain)
Functional Family Neutral protease 2

Enzyme Information

3.4.24.39
Deuterolysin.
based on mapping to UniProt P46076
Preferential cleavage of bonds with hydrophobic residues in P1'. Also 3-Asn-|-Gln-4 and 8-Gly-|-Ser-9 bonds in insulin B chain.
-!- Proteolytic activity found in Penicillium roqueforti, P.caseicolum, Aspergillus sojae and A.oryzae. -!- Optimum pH of 5 for digesting various proteins. -!- Strong action on protamine and histones. -!- Insensitive to phosphoramidon. -!- A distinct A.sojae endopeptidase is larger and has neutral pH optimum. -!- Belongs to peptidase family M35. -!- Formerly EC 3.4.24.4.

UniProtKB Entries (1)

P46076
NPII_ASPOR
Aspergillus oryzae RIB40
Neutral protease 2

PDB Structure

PDB 1EB6
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
A Quick Solution: Ab Initio Structure Determination of a 19 kDa Metalloproteinase Using Acorn
Mcauley, K.E., Jia-Xing, Y., Dodson, E.J., Lehmbeck, J., Ostergaard, P.R., Wilson, K.S.
Acta Crystallogr.,Sect.D