CATH Classification

Domain Context

CATH Clusters

Superfamily Clavaminate synthase-like
Functional Family

Enzyme Information

1.14.11.21
Clavaminate synthase.
based on mapping to UniProt Q05581
(1) Deoxyamidinoproclavaminate + 2-oxoglutarate + O(2) = amidinoproclavaminate + succinate + CO(2). (2) Proclavaminate + 2-oxoglutarate + O(2) = dihydroclavaminate + succinate + CO(2) + H(2)O. (3) Dihydroclavaminate + 2-oxoglutarate + O(2) = clavaminate + succinate + CO(2) + H(2)O.
-!- Catalyzes three separate oxidative reactions in the pathway for the biosythesis of the beta-lactamase inhibitor clavulanate in Streptomyces clavuligerus. -!- The first step (hydroxylation) is separated from the latter two (oxidative cyclization and desaturation) by the action of EC 3.5.3.22. -!- The three reactions are all catalyzed at the same nonheme iron site.

UniProtKB Entries (1)

Q05581
CAS1_STRC2
Streptomyces clavuligerus ATCC 27064
Clavaminate synthase 1

PDB Structure

PDB 1DS0
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structural origins of the selectivity of the trifunctional oxygenase clavaminic acid synthase.
Zhang, Z., Ren, J., Stammers, D.K., Baldwin, J.E., Harlos, K., Schofield, C.J.
Nat.Struct.Biol.