CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
2 | Mainly Beta |
|
2.60 | Sandwich |
|
2.60.120 | Jelly Rolls |
|
2.60.120.260 | Galactose-binding domain-like |
Domain Context
CATH Clusters
| Superfamily | Galactose-binding domain-like |
| Functional Family | YSIRK family gram-positive signal peptide |
Enzyme Information
| 3.2.1.97 |
Endo-alpha-N-acetylgalactosaminidase.
based on mapping to UniProt Q3T552
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H(2)O = 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein].
-!- The enzyme catalyzes the liberation of Gal-(1->3)-beta-GalNAc alpha- linked to serine or threonine residues of mucin-type glycoproteins. -!- EngBF from Bifidobacterium longum specifically acts on core 1-type O-glycan to release the disaccharide Gal-(1->3)-beta-GalNAc. -!- The enzymes from Clostridium perfringens, Enterococcus faecalis, Propionibacterium acnes and Alcaligenes faecalis show broader specificity (e.g. release of the core 2 trisaccharide Gal-(1->3)- beta-(GlcNAc-(1->6)-beta)-GalNAc or the core 3 disaccharide GlcNAc- (1->3)-beta-GalNAc). -!- The enzyme may play an important role in the degradation and utilization of mucins having core 1 O-glycan. -!- Formerly EC 3.2.1.110.
|
UniProtKB Entries (1)
| Q3T552 |
Q3T552_BIFL2
Bifidobacterium longum subsp. longum JCM 1217
Endo-alpha-N-acetylgalactosaminidase
|
PDB Structure
| PDB | 2ZXQ |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Crystallographic and mutational analyses of substrate recognition of endo-{alpha}-N-acetylgalactosaminidase from Bifidobacterium longum.
J.Biochem.
|