CATH Classification

Domain Context

CATH Clusters

Superfamily 1.10.8.50
Functional Family Endonuclease 8

Enzyme Information

3.2.2.-
Hydrolyzing N-glycosyl compounds.
based on mapping to UniProt P50465
4.2.99.18
DNA-(apurinic or apyrimidinic site) lyase.
based on mapping to UniProt P50465
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
-!- 'Nicking' of the phosphodiester bond is due to a lyase-type reaction, not hydrolysis. -!- This group of enzymes was previously listed as endonucleases, under the number EC 3.1.25.2.

UniProtKB Entries (1)

P50465
END8_ECOLI
Escherichia coli K-12
Endonuclease 8

PDB Structure

PDB 2OQ4
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Active site plasticity of endonuclease VIII: Conformational changes compensating for different substrates and mutations of critical residues
Golan, G., Zharkov, D.O., Grollman, A.P., Shoham, G.
To be Published