CATH Classification

Domain Context

CATH Clusters

Superfamily DNA helicase RuvA subunit, C-terminal domain
Functional Family Lipoyl synthase, mitochondrial

Enzyme Information

2.3.2.23
E2 ubiquitin-conjugating enzyme.
based on mapping to UniProt P61086
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine.
-!- The E2 ubiquitin-conjugating enzyme acquires the activated ubquitin from the E1 ubiquitin-activating enzyme (EC 6.2.1.45) and binds it via a transthioesterification reaction to itself. -!- In the human enzyme the catalytic center is located at Cys-87 where ubiquitin is bound via its C-terminal glycine in a thioester linkage. -!- Formerly EC 6.3.2.19.

UniProtKB Entries (1)

P61086
UBE2K_HUMAN
Homo sapiens
Ubiquitin-conjugating enzyme E2 K

PDB Structure

PDB 2O25
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
A Novel and Unexpected Complex Between the SUMO-1-Conjugating Enzyme UBC9 and the Ubiquitin-Conjugating Enzyme E2-25 kDa
Walker, J.R., Avvakumov, G.V., Xue, S., Newman, E.M., Mackenzie, F., Weigelt, J., Sundstrom, M., Arrowsmith, C.H., Edwards, A.M., Bochkarev, A., Dhe-Paganon, S.
To be Published