CATH Classification

Domain Context

CATH Clusters

Superfamily Alpha-ketoglutarate-dependent dioxygenase AlkB-like
Functional Family Alkylated DNA repair protein AlkB

Enzyme Information

1.14.11.33
DNA oxidative demethylase.
based on mapping to UniProt P05050
DNA-base-CH(3) + 2-oxoglutarate + O(2) = DNA-base + formaldehyde + succinate + CO(2).
-!- Activity is slightly stimulated by ascorbate. -!- Catalyzes oxidative demethylation of the DNA base lesions N(1)- methyladenine, N(3)-methylcytosine, N(1)-methylguanine, and N(3)- methylthymine. -!- It works better on single-stranded DNA (ssDNA) and is capable of repairing damaged bases in RNA.
1.14.11.-
With 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors.
based on mapping to UniProt P05050

UniProtKB Entries (1)

P05050
ALKB_ECOLI
Escherichia coli K-12
Alpha-ketoglutarate-dependent dioxygenase AlkB

PDB Structure

PDB 2FDF
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Crystal structures of catalytic complexes of the oxidative DNA/RNA repair enzyme AlkB.
Yu, B., Edstrom, W.C., Benach, J., Hamuro, Y., Weber, P.C., Gibney, B.R., Hunt, J.F.
Nature