CATH Classification

Domain Context

CATH Clusters

Superfamily Bira Bifunctional Protein; Domain 2
Functional Family Lipoate-protein ligase a related

Enzyme Information

6.3.1.20
Lipoate--protein ligase.
based on mapping to UniProt Q9HKT1
ATP + (R)-lipoate + a [lipoyl-carrier protein]-L-lysine = a [lipoyl- carrier protein]-N(6)-(lipoyl)lysine + AMP + diphosphate.
-!- This enzyme participates in lipoate salvage, and is responsible for lipoylation in the presence of exogenous lipoic acid. -!- The enzyme attaches lipoic acid to the lipoyl domains of certain key enzymes involved in oxidative metabolism, including pyruvate dehydrogenase (E(2) domain), 2-oxoglutarate dehydrogenase (E(2) domain), the branched-chain 2-oxoacid dehydrogenases and the glycine cleavage system (H protein). -!- Lipoylation is essential for the function of these enzymes. -!- The enzyme can also use octanoate instead of lipoate. -!- Formerly EC 2.7.7.63.

UniProtKB Entries (1)

Q9HKT1
LPLAN_THEAC
Thermoplasma acidophilum DSM 1728
Lipoate-protein ligase A subunit 1

PDB Structure

PDB 2C8M
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structure of a Putative Lipoate Protein Ligase from Thermoplasma Acidophilum and the Mechanism of Target Selection for Post-Translational Modification.
Mcmanus, E., Luisi, B.F., Perham, R.N.
J.Mol.Biol.