CATH Classification

Domain Context

CATH Clusters

Superfamily Tetratricopeptide repeat domain
Functional Family Prolyl 4-hydroxylase subunit alpha 2

Enzyme Information

1.14.11.2
Procollagen-proline 4-dioxygenase.
based on mapping to UniProt O15460
L-proline-[procollagen] + 2-oxoglutarate + O(2) = trans-4-hydroxy-L- proline-[procollagen] + succinate + CO(2).
-!- The enzyme, which is located within the lumen of the endoplasmic reticulum, catalyzes the 4-hydroxylation of prolines in -X-Pro-Gly- sequences. -!- The 4-hydroxyproline residues are essential for the formation of the collagen triple helix. -!- The enzyme forms a complex with protein disulfide isomerase and acts not only on procollagen but also on more than 15 other proteins that have collagen-like domains.

UniProtKB Entries (1)

O15460
P4HA2_HUMAN
Homo sapiens
Prolyl 4-hydroxylase subunit alpha-2

PDB Structure

PDB 6EVL
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural enzymology binding studies of the peptide-substrate-binding domain of human collagen prolyl 4-hydroxylase (type-II): High affinity peptides have a PxGP sequence motif.
Murthy, A.V., Sulu, R., Koski, M.K., Tu, H., Anantharajan, J., Sah-Teli, S.K., Myllyharju, J., Wierenga, R.K.
Protein Sci.