CATH Classification

Domain Context

CATH Clusters

Superfamily 3.30.1360.170
Functional Family Flavin-dependent thymidylate synthase

Enzyme Information

2.1.1.148
Thymidylate synthase (FAD).
based on mapping to UniProt Q9WYT0
5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP(+).
-!- Contains FAD. -!- All thymidylate synthases catalyze a reductive methylation involving the transfer of the methylene group of 5,10-methylenetetrahydrofolate to the C5-position of dUMP and a two electron reduction of the methylene group to a methyl group. -!- Unlike the classical thymidylate synthase, ThyA (EC 2.1.1.45), which uses folate as both a 1-carbon donor and a source of reducing equivalents, this enzyme uses a flavin coenzyme as a source of reducing equivalents, which are derived from NADPH.

UniProtKB Entries (1)

Q9WYT0
THYX_THEMA
Thermotoga maritima MSB8
Flavin-dependent thymidylate synthase

PDB Structure

PDB 5IOR
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Deprotonations in the Reaction of Flavin-Dependent Thymidylate Synthase.
Stull, F.W., Bernard, S.M., Sapra, A., Smith, J.L., Zuiderweg, E.R., Palfey, B.A.
Biochemistry