CATH Classification

Domain Context

CATH Clusters

Superfamily alpha/beta hydrolase
Functional Family putative methylesterase 11, chloroplastic

Enzyme Information

3.1.1.82
Pheophorbidase.
based on mapping to UniProt O23512
Pheophorbide a + H(2)O = pyropheophorbide a + methanol + CO(2).
-!- Forms part of the chlorophyll degradation pathway, and is found in higher plants and in algae. -!- In higher plants it participates in de-greening processes such as fruit ripening, leaf senescence, and flowering. -!- Exists in two forms: type 1 is induced by senescence whereas type 2 is constitutively expressed. -!- Highly specific for pheophorbide as substrate (with a preference for pheophorbide a over pheophorbide b) as other chlorophyll derivatives such as protochlorophyllide a, pheophytin a and c, chlorophyll a and b, and chlorophyllide a cannot act as substrates. -!- Another enzyme, called pheophorbide demethoxycarbonylase (PDC), produces pyropheophorbide a from pheophorbide a without forming an intermediate although the precise reaction is not yet known.

UniProtKB Entries (1)

O23512
PPD_ARATH
Arabidopsis thaliana
Probable pheophorbidase

PDB Structure

PDB 5HK8
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Crystal strucutre of a methylesterase protein MES16 from Arabidopsis
Li, H., Pu, H.
Biochem.Biophys.Res.Commun.