CATH Classification

Domain Context

CATH Clusters

Superfamily Nitric Oxide Synthase;Heme Domain;Chain A domain 2
Functional Family

Enzyme Information

1.14.13.39
Nitric-oxide synthase (NADPH).
based on mapping to UniProt P29475
2 L-arginine + 3 NADPH + 4 O(2) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O.
-!- The enzyme consists of linked oxygenase and reductase domains. -!- The eukaryotic enzyme binds FAD, FMN, heme (iron protoporphyrin IX) and tetrahydrobiopterin, and its two domains are linked via a regulatory calmodulin-binding domain. -!- Upon calcium-induced calmodulin binding, the reductase and oxygenase domains form a complex, allowing electrons to flow from NADPH via FAD and FMN to the active center. -!- The reductase domain of the enzyme from the bacterium Sorangium cellulosum utilizes a [2Fe-2S] cluster to transfer the electrons from NADPH to the active center. -!- Cf. EC 1.14.14.47.

UniProtKB Entries (1)

P29475
NOS1_HUMAN
Homo sapiens
Nitric oxide synthase, brain

PDB Structure

PDB 5FVX
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Potent and Selective Human Neuronal Nitric Oxide Synthase Inhibition by Optimization of the 2-Aminopyridine-Based Scaffold with a Pyridine Linker.
Wang, H., Qin, Y., Li, H., Roman, L.J., Martasek, P., Poulos, T.L., Silverman, R.B.
J.Med.Chem.