CATH Classification

Domain Context

CATH Clusters

Superfamily 6.10.240.10
Functional Family

Enzyme Information

1.1.1.86
Ketol-acid reductoisomerase (NADP(+)).
based on mapping to UniProt C1DFH7
(1) (2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-hydroxy-2- methyl-3-oxobutanoate + NADPH. (2) (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-hydroxy-2- ethyl-3-oxobutanoate + NADPH.
-!- The enzyme, found in many bacteria and archaea, is specific for NADPH (cf. EC 1.1.1.382 and EC 1.1.1.383). -!- Formerly EC 1.1.1.89.

UniProtKB Entries (1)

C1DFH7
ILVC_AZOVD
Azotobacter vinelandii DJ
Ketol-acid reductoisomerase (NADP(+))

PDB Structure

PDB 4XIY
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Cofactor specificity motifs and the induced fit mechanism in class I ketol-acid reductoisomerases.
Cahn, J.K., Brinkmann-Chen, S., Spatzal, T., Wiig, J.A., Buller, A.R., Einsle, O., Hu, Y., Ribbe, M.W., Arnold, F.H.
Biochem.J.