CATH Classification

Domain Context

CATH Clusters

Superfamily Cysteine proteinases
Functional Family Ubiquitin carboxyl-terminal hydrolase 8

Enzyme Information

3.4.19.12
Ubiquitinyl hydrolase 1.
based on mapping to UniProt P50102
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
-!- Links to polypeptides smaller than 60 residues are hydrolyzed more readily than those to larger polypeptides. -!- Isoforms exist with quantitatively different specificities among the best known being UCH-L1 and UCH-L3, major proteins of the brain of mammals. -!- Inhibited by ubiquitin aldehyde (in which Gly76 is replaced by aminoacetaldehyde). -!- Belongs to peptidase family C12.

UniProtKB Entries (2)

A6ZWK1
SGF11_YEAS7
Saccharomyces cerevisiae YJM789
SAGA-associated factor 11
P50102
UBP8_YEAST
Saccharomyces cerevisiae S288C
Ubiquitin carboxyl-terminal hydrolase 8

PDB Structure

PDB 4WA6
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structure of yeast SAGA DUBm with Sgf73 N59D mutant at 2.36 angstroms resolution
Wolberger, C., Yan, M.
To Be Published