CATH Classification

Domain Context

CATH Clusters

Superfamily Lyase 2-enoyl-coa Hydratase, Chain A, domain 2
Functional Family Enoyl-CoA delta isomerase 2, mitochondrial

Enzyme Information

5.3.3.8
Delta(3)-Delta(2)-enoyl-CoA isomerase.
based on mapping to UniProt O75521
(1) A (3Z)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA. (2) A (3E)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.
-!- The enzyme participates in the beta-oxidation of fatty acids with double bonds at an odd position. -!- Processing of these substrates via the beta-oxidation system results in intermediates with a cis- or trans-double bond at position C(3), which cannot be processed further by the regular enzymes of the beta- oxidation system. -!- This enzyme isomerizes the bond to a trans bond at position C(2), which can be processed further. -!- The reaction rate is ten times higher for the (3Z) isomers than for (3E) isomers. -!- The enzyme can also catalyze the isomerization of 3-acetylenic fatty acyl thioesters to 2,3-dienoyl fatty acyl thioesters.

UniProtKB Entries (1)

O75521
ECI2_HUMAN
Homo sapiens
Enoyl-CoA delta isomerase 2, mitochondrial

PDB Structure

PDB 4U19
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Human Delta (3) , Delta (2) -enoyl-CoA isomerase, type 2: a structural enzymology study on the catalytic role of its ACBP domain and helix-10.
Onwukwe, G.U., Kursula, P., Koski, M.K., Schmitz, W., Wierenga, R.K.
Febs J.